Principles Of Three-dimensional Imaging In Confocal Microscopes

Min Gu

• Publisher: World Scientific
• Year: 1996
• Print ISBN: 9789810225506, 9789810225506
• eBook ISBN: 9789814261104
• Topic: Biological Sciences
• Subtopic: Science General
• Index: Biological Sciences

This book discusses the various principles in confocal scanning microscopy which has become a useful tool in many practical fields including biological studies and industrial inspection. The methodology presented in this book is unique and is based on the concept of the three-dimensional transfer functions which have been developed by the author and his colleagues over the last five years. With the 3-D transfer functions, resolving power in 3-D confocal imaging can be defined in a unified way, different optical arrangements can be compared with an insight into their inter-relationship, and images of thick objects can be modeled in terms of the Fourier transform which makes the analysis easy. The aim of this book is to provide a systematic introduction to the concept of the 3-D transfer functions in various confocal microscopes, to describe the methods for the derivation of different 3-D transfer functions, and to explain the principles of 3-D confocal imaging in terms of these functions.

Table of contents

• Contents
• Preface
• Chapter 1. Introduction
• Chapter 2. Three-Dimensional Fourier Optics of a Thin Lens
• Chapter 3. Confocal Bright-Field Microscopy with a Point Detector
• Chapter 4. Confocal Bright-Field Microscopy with a Finite-Sized Detector
• Chapter 5. Confocal Single-Photon Fluorescence Microscopy
• Chapter 6. Confocal Two-Photon Fluorescence Microscopy
• Chapter 7. Fibre-Optical Confocal Microscopy
• Chapter 8. Confocal Microscopy under Ultrashort Pulse Illumination
• Chapter 9. Confocal Microscopy with High-Aperture Objectives
• Chapter 10. Significance of Three-Dimensional Transfer Functions
• Appendix 1 Hankel Transform
• Appendix 2 List of Symbols
• Appendix 3 Abbreviation
• Subject Index