Culture Techniques

Applicability for Studies on Prenatal Differentiation and Toxicity. Fifth Symposium on Prenatal Development, May 1981, Berlin

Diether Neubert, Hans J. Merker, J. Klein-Friedrich, R. Kreft, Diether Neubert, Hans J. Merker, J. Klein-Friedrich, R. Kreft

• Éditeur: De Gruyter
• Année: 2019
• Imprimer l'ISBN:
  9783110087543
• ISBN de l'eBook:
  9783110858242
• Sujet: Medicine
• Sujet: Gynecology, Obstetrics & Midwifery

No detailed description available for "Culture Techniques".

Table des matières

• Dedication
• Preface
• Table of Contents
• Introduction
• I. Studies on Whole-Embryo Culture
• Differentiation of Embryonic Tissues in Whole-Embryo Cultures as Compared to the Development In Vivo
• The Differentiation Between Teratogenic Action on Mother, Fetal Membranes and Embryos Using the Whole-Embryo Culture Method
• Trypan Blue Teratogenesis in the Rat In Vitro
• Studies on Embryotoxic Effects of Thallium Using the Whole-Embryo Culture Technique
• Evaluation of Serum Teratogenic Activity Using Rat Embryo Cultures
• Regulation of Mouse Embryogenesis by Exogenous Growth Factors
• Preliminary Communication on the Feasibility of Culturing Whole Embryos in a Chemically Defined Medium
• In Vitro Development of the Heart Under Influence of Retinole Acid
• II. Studies on Organ Cultures
• A. Differentiation of the Skeleton (predominantly Extremities)
• Simulation of Limb Bud Skeletogenesis In Vitro
• Comparison of the Differentiation of Muscle and Connective Tissue of Mouse Limb Buds in Culture and In Vivo: A Morphological Study by Indirect Immunofluorescence
• Quantification of Collagen Types I and II in Mouse Limbs during Differentiation In Vitro and In Vivo
• Feasibility of Storing Embryonic Tissues for Subsequent Use in Organ Culture
• Limb Bud Organ Cultures from Mouse Embryos after Apparent Induction of Monooxygenases In Utero. Effects of Cyclophosphamide, Dimethylnitrosamine and Some Thalidomide Derivatives
• On the Significance of Ascorbate and of Cysteine on Differentiation of Limb Buds in Organ Culture
• Effects of Some "Indirectly" Alkylating Agents on Differentiation of Limb Buds in Organ Culture
• Comparison of Effects on Limb Development In Vivo and In Vitro Using Methyl(acetoxymethyl)nitrosamine
• Development of Limb Buds in Organ Culture: Examination of Hydroxyurea Enhancement of Bromodeoxyuridine Toxicity Using Image Analysis
• Biochemical Characterization of Mouse Hereditary Chondrodystrophies in Organ Culture
• Effect of Teratogens on Human Embryonic Skeletal Tissue In Vitro
• Aspirin Induced Polydactyly in Rats Mediated by Inhibition of Prostaglandin Synthesis
• B. Differentiation of other Organ Anlagen (kidneys, lungs, heart, prostate gland, haematopoetic system, and intestine)
• The Developing Kidney as a Model System for Normal and Impaired Organogenesis
• The Embryonic Lung as In Vitro Model for Testing Teratogenic Substances
• The Toxic Effects of Metal Dusts on Human Foetal Lungs In Vitro
• Effects of Substances Influencing Glycosaminoglycan Synthesis on Lung Development in Culture
• Effects of L-azetidine-2-carboxylic Acid and ß-D-xylosides on Lung Development In Vitro
• Interaction of Epithelium and Mesenchyme in the Induction of Foetal Rat and Mouse Prostate Glands by Androgens in Organ Culture
• In Vivo and In Vitro Assays of Trisomie Cells Isolated from the Fetal Organism or Rescued by Transfer to Non-Trisomic Hosts
• Effects of Gliadin-derived Peptides from Bread and Durum Wheat on Small Intestine Cultures from Rat Fetus and Coeliac Children
• III. Studies on Preimplantation Embryos
• Differential Adhesiveness as a Mechanism of Cell Allocation to Inner Cell.Mass and Trophectoderm in the Mouse Blastocyst
• Inefficient Capacity for Oxidative Phosphorylation of the Trophoblast as a Cause of Delayed Implantation in the Mouse
• Influence of Micromanipulation of Ova on the Postnatal Development of Mice (AKR)
• Investigations on the Mechanism of Action and on the Pharmacokinetics of Cyclophosphamide Treatment during the Preimplantation Period in the Mouse
• Benzo(a)pyrene Metabolism in Early Mouse Embryos
• IV. Studies on Cell and Tissue Cultures
• Influence of Different Antisera on Mouse Epiphyseal Chondrocytes in Monolayer Culture
• Cell Movement during Blastema Formation. Effect of Cytochalasin B on Cartilage Development In Vitro
• Cell-Substrate Adhesion of Fibroblast-like Cells Under Different In Vitro Conditions. A Scanning and Transmission Electron-Microscopic Study
• Development of Cytochrome P-450-Dependent Drug Metabolizing Enzyme Activities in Mouse and Human Tissues In Vitro
• In Vitro Treatment with Metallocene Dichlorides: Determination of the Intracellular Distribution of the Metal Atoms by Use of the Electron Energy Loss Spectroscopy
• Morphological Changes Induced by 2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) in Epithelial Monolayer Culture Using Human and Rodent Fetal Tissues
• V. Studies on Non-mammalian Tissues
• The Chick Embryo: A Standard Against Which to Judge In Vitro Systems
• Experimental Manipulation Leading to Cardiac Malformation in Chick Embryo
• Morphogenese Systems and In Vitro Techniques in Teratology
• VI. Culture Techniques for "Screening" on Teratogenicity
• An Assessment of the Available In Vitro Techniques for Detecting Teratogens
• On the Predictability of Developmental Toxicity -especially Prenatal Toxicity - On the Basis of Culture Experiments
• VII. Some Methods Used for Culturing Embryonic Tissues
• A. Whole-Embryo-Culture: Beck; Anschiitz
• B. Limb Bud Method: Blankenburg
• C. Trowell Method: Zimmermann
• D. "Micro-Mass Culture": Zimmermann
• E. Preimplantation-Embryo Culture
• F. Teratogenicity Testing Method (Chick)
• G. CHEST I and II (Chick): Jelinek and Peterka
• Glossary
• List of Participants
• Index