PCR: Methods Express
eBook - PDF

PCR: Methods Express

  1. 348 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

PCR: Methods Express

About this book

PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR
*rapid generation of gene targeting constructs
*PCR multiplexes
*PCR-based mutagenesis
*identification of microdeletions and microduplications*DNA methylation analysis
*forensic genetic DNA typing
*genotyping
*identification of mutations in single cells
*whole genome amplification
*diagnosis of infectious diseases
*inverse PCR-based RFLPThis book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator.

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Yes, you can access PCR: Methods Express by Simon Hughes,Adrian Moody in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Molecular Biology. We have over one million books available in our catalogue for you to explore.

Table of contents

  1. Contents
  2. Color Section
  3. CHAPTER 1: An introduction to the polymerase chain reaction
  4. CHAPTER 2: Polymerases for PCR
  5. CHAPTER 3: A detailed guide to quantitative RT-PCR
  6. CHAPTER 4: Use of quantitative PCR for the detection of genomic microdeletions or microduplications
  7. CHAPTER 5: Robust and unique PCR for single-nucleotide polymorphism genotyping applications
  8. CHAPTER 6: Using PCR and linkage mapping to identify single genes and quantitative trait loci for livestock traits
  9. CHAPTER: 7 PCR restriction fragment length polymorphism analysis for genotyping of single-nucleotide polymorphisms
  10. CHAPTER 8: Forensic genetic DNA typing with PCR-based methods
  11. CHAPTER 9: Large PCR multiplexes with special reference to forensic single-nucleotide polymorphism typing
  12. CHAPTER 10: Pre-implantation genetic diagnosis of monogenic disease...
  13. CHAPTER 11: Rapid generation of gene-targeting constructs
  14. CHAPTER 12: Construction of long DNA molecules from multiple fragments using PCR
  15. CHAPTER 13: Efficient PCR-based mutagenesis method applicable to diverse mutagenesis strategies using type IIs restriction enzymes
  16. CHAPTER 14: Inverse PCR-based restriction fragment length polymorphism for identifying low-level mutations in tumors
  17. CHAPTER 15: PCR methods for infectious disease diagnosis
  18. CHAPTER 16: Use of PCR for DNA methylation analyses
  19. CHAPTER 17: PCR-based methods to determine DNA methylation status...
  20. CHAPTER 18: PCR-based whole genome amplification
  21. CHAPTER 19: PCR sequencing of human genes for the discovery of DNA sequence variants
  22. APPENDIX 1 List of suppliers
  23. Index