Overview of blotting techniques
eBook - PDF

Overview of blotting techniques

  1. 255 pages
  2. English
  3. PDF
  4. Available on iOS & Android
eBook - PDF

Overview of blotting techniques

About this book

There are 2 techniques that are commonly employed in research labs: gel electrophoresis and blotting. Following electrophoresis, separated nucleic acids and proteins can be transferred to a membrane where they are analyzed or detected. Depending on the molecule being analyzed, Southern blot refers to transfer and studying DNA, Northern blotting refers to RNA, Western blotting to proteins and eastern blotting for post-translational modifications. Given the importance of blotting in the lab, this book comes at an opportune time. The book discusses the protocols and applications of blotting-Southern, western, northern and eastern blotting. Case studies have also presented to help the reader gain insights into the practicality and applicability of blotting in today's world. The editors hope that this book will serve as a lab staple just like the aforementioned blotting techniques.

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Information

Publisher
Delve Publishing
Year
2020
Topic
Biological Sciences
eBook ISBN
9781774078945
Subtopic
Biology
Index
Biological Sciences

Table of contents

  1. Cover
  2. Copyright
  3. DECLARATION
  4. ABOUT THE EDITORS
  5. TABLE OF CONTENTS
  6. List of Contributors
  7. List of Abbreviations
  8. Preface
  9. Chapter 1 Western Blotting-the Works
  10. Chapter 2 Southern Blotting and Associated Techniques
  11. Chapter 3 Northern Blotting: A Brief Overview
  12. Chapter 4 Eastern Blotting
  13. Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures
  14. Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract
  15. Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data
  16. Chapter 8 The Design of A Quantitative Western Blot Experiment
  17. Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis
  18. Chapter 10 Sensitive Detection Of Fluorescence In Western Blotting by Merging Images
  19. Chapter 11 Total Protein Analysis As A Reliable Loading Control For Quantitative Fluorescent Western Blotting
  20. Index
  21. Back Cover

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Yes, you can access Overview of blotting techniques by Shiv Sanjeevi Sripathi,Prerna Pandey, Shiv Sanjeevi Sripathi, Prerna Pandey in PDF and/or ePUB format, as well as other popular books in Biological Sciences & Biology. We have over 1.5 million books available in our catalogue for you to explore.