Electrofocusing and Isotachophoresis

Proceedings of the International Symposium, August 1–4, 1976, Hamburg, Germany

B. J. Radola, D. Graesslin, B. J. Radola, D. Graesslin

• Verlag: De Gruyter
• Jahr: 2019
• ISBN drucken:
  9783110070262
• eBook-ISBN:
  9783110873870
• Thema: Biowissenschaften
• Thema: Biochemie

Keine ausführliche Beschreibung für "Electrofocusing and Isotachophoresis" verfügbar.

Inhaltsverzeichnis

• Preface
• Contents
• Contributors
• Part I. Isoelectric Focusing
• 1. Theoretical and General Aspects
• Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea
• Stable pH Gradients - A Key Problem in Isoelectric Focusing
• Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential
• Comparison of pH Gradients Used for Isoelectric Focusing
• On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing
• 2. Carrier Ampholytes
• Carrier Ampholyte Distribution
• Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods
• Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range
• 3. Analytical Methodology
• Staining of Proteins in Polyacrylamide Gels
• Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF
• Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing
• Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry
• Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins
• A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis
• pH Determinations in Isoelectric Focusing with an Iridium Electrode
• Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing
• Does Lipoprotein Lipase Bind Ampholytes?
• 4. Biochemical Applications
• Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation
• Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems
• A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing
• Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae)
• Fractionation of Nucleic Acids on Isoelectric Focusing
• 5. Clinical Applications
• Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (α₁Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies
• Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (α₁Pi) Phenotyping: II. Immunochemical Procedures
• High Resolution Phenotyping of α₁-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing
• Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation
• Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing
• The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing
• Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality
• Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing
• Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay
• Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing
• A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing
• 6. Two-Dimensional Techniques
• Biological Applications of Two-Dimensional Gel Electrophoresis
• Plant Proteins Evaluated by Two-Dimensional Methods
• Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis
• Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins
• Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens
• 7. Preparative Separations
• Isoelectric Focusing in Free Water
• Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects
• Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis
• Preparative Zone Convection Electrofocusing in Helical Glass Tubes
• Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential
• 8. Separation of Cells
• New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques
• Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction
• Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient
• Part II. Isotachophoresis
• 1. Analytical and Preparative Methodology
• The Principle of Preparative Capillary Isotachophoresis
• Preparative Capillary Isotachophoresis: Separation of μg Amounts of Some Human Serum Proteins
• Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis
• Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing
• Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization
• 2. Applications
• Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers
• Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis
• Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies
• Index