Oxidative Eustress in Exercise Physiology

James N. Cobley, Gareth W. Davison, James N. Cobley, Gareth W. Davison

• Verlag: CRC Press
• Jahr: 2022
• ISBN:
  9781000557336
• Auflage: 1
• Thema: Scienze biologiche
• Thema: Biochimica

Oxidative Eustress in Exercise Physiology unravels key physiological responses and adaptations to different redox-regulated exercise paradigms at the cell, tissue, and whole-body level in model systems and humans in health and disease. While the mechanistic details are still unclear, key intracellular redox indices seem to be dysregulated with age. Consequently, beneficial molecular responses to acute endurance exercise decline in older individuals. Recent research suggests that manipulating mitochondrial redox homeostasis by supplementing with the mitochondria-targeted coenzyme Q10 for six weeks markedly improves physical function in older adults; i.e. it may be possible to maximise the benefits of exercise by manipulating the redox environment. The research described in this book suggests that significant translational potential exists with respect to cardiovascular disease, neurodegeneration and cancer. An international team of researchers documents the importance of redox biology in health and disease, especially when exercise is a clinically useful tool for the treatment of many diseases and conditions.

Features

• Defines essential redox biology reactions and concepts in exercise physiology

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• Assesses key redox parameters in an in vivo human exercise context

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• Identifies the challenges, opportunities and boundaries of current knowledge

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• Includes a critique of the underlying mechanisms

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• Summarises examples of translationally important research relating to disease states

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Related Titles

Draper, N. & H. Marshall. Exercise Physiology for Health and Sports Performance (ISBN 978-0-2737-7872-1)

Wackerhage, H., ed. Molecular Exercise Physiology: An Introduction (ISBN 978-0-4156-0788-9)

CHAPTER ONE Introduction to Oxidative (Eu)stress in Exercise Physiology

Gareth W. Davison and James N. Cobley

DOI: 10.1201/9781003051619-1

CONTENTS

1. Introduction
2. Oxygen and Its Derivatives
3. Superoxide Anion
4. Hydrogen Peroxide
5. Hydroxyl Radical
6. Reactive Nitrogen Species
7. Brief Overview of Indirect and Direct Biomarkers of Exercise-Induced Oxidative Stress
8. Brief Overview of ROS as Eustress Regulators of Skeletal Muscle
9. Conclusion
10. Acknowledgement
11. References

Introduction

Exercise-induced oxidative (eu)stress has received considerable attention over the last four decades, with circa 900 original investigations published since 1978. Indeed, redox research per se is a contemporary feature of biomedical journals and conferences, with a plethora of exciting new information relating to health and disease being presented.

The discovery that free radicals (i.e., molecules capable of existing independently, and containing one or more unpaired electrons) exist in living biological organisms occurred in 1954 when Commoner and colleagues (Commoner et al., 1954) first used electron paramagnetic resonance (EPR) spectroscopy to detect free radicals in growing seeds. Around this time, Gerschman et al. (1954) proposed that free radicals damage cells, and second, when mice are exposed to hyperoxia, the biological injury that ensues is likely related to increased free radical production. Following the publication of this work in the mid-1950s, a series of seminal studies confirmed the biological importance (Powers et al., 2016). For example, the work of Chance and Williams (1956) demonstrated that respiring mitochondria can generate hydrogen peroxide, which can diffuse in cells. Then, McCord and Fridovich (1969) discovered the metalloenzyme superoxide dismutase (SOD), showing in the process that superoxide radicals are spontaneously degraded to hydrogen peroxide. The milestone discovery of SOD not only provided much of the basis of our current understanding of antioxidant defence systems, but it is also credited with providing the first convincing evidence that biological reactive oxygen species (ROS) exist and that they are likely salient regulators of cell biology (Powers et al., 2016).

Since these landmark discoveries, the exercise physiology and redox field have grown exponentially with prominent work enhancing our understanding of the role that free radicals (e.g., superoxide) and non-radicals (e.g., hydrogen peroxide), collectively termed ROS, play in systemic and intracellular muscle biology. It is now known, for example, that both different exercise modalities generate ROS and their production is associated with molecular biomarkers of oxidative stress. To the contrary, exercise-induced low-levels of ROS activate key signalling pathways leading to exercise-induced skeletal muscle adaptations. This chapter is designed to introduce the basic concept of oxidative (eu)stress, and in doing so, a brief overview of the key studies pertaining to exercise-induced oxidative stress and those identified as eustress regulators of skeletal muscle will be highlighted. A brief introduction to ground state molecular dioxygen and its derivatives (i.e., ROS) will also provide context to the general area of interest.

‘Oxidative stress’ is a popular term among exercise physiologists and is widely used in the literature, but rarely defined. Sies and Cadenas (1985) defined the term oxidative stress as ‘a disturbance in the prooxidant–antioxidant balance in favour of the former’. Although this definition was widely accepted for over two decades, this description of oxidative stress has undergone scrutiny (Powers et al., 2016), and subsequently, Jones and Sies revised the definition of oxidative stress to ‘an imbalance between oxidants and antioxidants in favour of the oxidants, leading to a disruption of redox signalling and control and/or molecular damage’ (Sies & Jones, 2007). On the latter, the basic premise is that, in an open metabolic system, a steady-state redox balance is maintained at a particular setpoint, providing a basal redox tone, and any deviation away from the steady-state redox balance is regarded as a stress response. This oxidative distress concept, which explains supraphysiological disruption to redox signalling and/or oxidative stress damage to biomolecules, however, only explains one end of the continuum. At the other end, the term oxidative eustress may be used to connect low levels of oxidative stress to intracellular redox signalling and regulation (Sies 2020a).

Oxygen and Its Derivatives

Arguably the most important free radicals in biological systems are radical derivatives from oxygen, and although oxygen is necessary for survival, it has the potential to become toxic when supplied at concentrations higher than normally encountered (i.e., the toxicity of molecular oxygen is primarily due to the production of ROS). Ground state molecular oxygen is, in fact, a di-radical, with two unpaired electrons located in a different antibonding orbital with the same directional spin. Consequently, oxygen can only react with non-radicals by accepting a pair of electrons that spin in an anti-parallel manner (McCord, 1979).

Free radicals in biological organisms include, but not limited to superoxide (O₂^.−), hydroperoxyl (HO₂^.), hydroxyl (OH^.), carbonate (CO₃^.−), peroxyl (RO₂^.), alkoxyl (RO^.) and nitric oxide (NO) radicals (Table 1.1). Hydrogen peroxide (H₂O₂) and hypochlorous acid (HOCL) have no unpaired electrons, and by definition they are non-radicals, but nevertheless these ROS can be powerful oxidants that are often involved in free radical reactions.

TABLE 1.1 Reactive Oxygen Species Present in Biological Systems

Free Radical	Chemical Formula	Half-Life (seconds)
Hydroxyl	${\text{OH}}^{\cdot }$	1 × 10^−9
Superoxide	${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$	1 × 10^−6
Singlet oxygen	^1O2	1 × 10^−6
Alkoxyl	${\text{RO}}^{\cdot }$	1 × 10^−6
Molecular oxygen	O2	> 10^2
Hydrogen peroxide	H2O2	10
Peroxyl	${\text{ROO}}^{\cdot }$	17
Hydroperoxyl	${{\displaystyle {\text{HO}}_{\text{2}}}}^{\cdot }$	?
Carbonate	${{\displaystyle {\text{CO}}_{\text{3}}}}^{\cdot -}$	?
Semiquinone	${\text{Q}}^{\cdot -}$	Days

Superoxide Anion

Superoxide $\left({{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}\right)$ is a commonly known oxygen-centred free radical. The reduction of a single electron to an O₂ molecule produces the superoxide anion. ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ is relatively unreactive with non-radical species in comparison to other radical types (e.g., hydroxyl radical); however, if ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ is generated near the site of any biochemical molecule it can be damaging. The reactivity of ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ in aqueous solutions is more likely to occur in vivo (Halliwell and Gutteridge, 2015). In this environment, ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ can act as a base, accepting a proton to form the hydroperoxyl radical (HO₂^.). The pKa for this reaction is 4.8, indicating that approximately only 1% of ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ is in the ${{\displaystyle {\text{HO}}_{\text{2}}}}^{\cdot }$ form at physiological pH (Pryor, 1986). However, there may be more ${{\displaystyle {\text{HO}}_{\text{2}}}}^{\cdot }$ at comparatively acidic membrane nanodomains. ${{\displaystyle {\text{O}}_{\text{2}}}}^{\cdot -}$ under a normal metabolic response is dismutated by SOD, which can increase the rate of intracellular dismutation by a factor of 10⁻⁹ M⁻¹s⁻¹ to form hydrogen peroxide...